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Anti-MASH1/Achaete-scute homolog 1 antibody
Anti-MASH1/Achaete-scute homolog 1 antibody from Abcam
Be the first to write a review!
Citations:
(36)
Supplier Page
Supplier Page from
Abcam for
Anti-MASH1/Achaete-scute homolog 1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-MASH1/Achaete-scute homolog 1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab74065
Antibody Type
Primary
Quantity
100 UG
Conjugate/Tag
Unconjugated
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Host
Rabbit
Isotype
IgG
Concentration
0.3 mg/ml
Gene Name
Ascl1
Reactivity
Human, Mouse, Rat, Zebrafish
Target
MASH1/Achaete-scute homolog 1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (36)
(1)
Joint sequence & chromatin neural networks characterize the differential abilities of Forkhead transcription factors to engage inaccessible chromatin
bioRxiv
November 5, 2023
Sonny Arora, Jianyu Yang, Tomohiko Akiyama, Daniela Q. James, Alexis Morrissey, Thomas R. Blanda, Nitika Badjatia, William K.M. Lai, Minoru S.H. Ko, B. Franklin Pugh,
et al.
SLBW5142), anti-IgG (
i5381
Sigma
, lot SLBQ7097V), anti-Foxa1 (
Abcam
ab23738
, lot GR3241520-1), anti-Ascl1 (
ab74065
Abcam
, lot GR3209594-1). Null control
ChIP-exo
experiments were performed using the anti-FLAG-M2 antibody
(2)
Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke
Nature Communications
October 10, 2023
Michael R. Williamson, Stephanie P. Le, Ronald L. Franzen, Nicole A. Donlan, Jill L. Rosow, Mathilda S. Nicot-Cartsonis, Alexis Cervantes, Benjamin Deneen, Andrew K. Dunn, Theresa A. Jones,
et al.
BrdU. Primary antibodies and dilutions were as follows: Rabbit polyclonal anti-ASCL1 (
1:1000
)
Abcam
ab74065
, Rabbit polyclonal anti-ASCL1 (
1:500
)
Cosmo Bio
CAC-SK-T01-003
, Rabbit polyclonal anti-CD133 (
1:1000
)
(3)
Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalon
Neural Development
September 8, 2023
Mary Jo Talley, Diana Nardini, Lisa A. Ehrman, Q. Richard Lu, Ronald R. Waclaw
following concentrations: Animal-Antibody (Dilution, Company, catalog #), Rbt-Ascl1 (
1:5,000
Abcam
, #
AB74065
), Gt-βgal (
1:1,000
, Biogenesis, #2282), Rbt-βgal (
1:1000
, Biogenesis, #4600 − 1509),
Rat
-BrdU (
1:250
,
(4)
Glycolysis mediates neuron specific histone acetylation in valproic acid-induced human excitatory neuron differentiation
Frontiers in molecular neuroscience
January 1, 2023
Chen A, Wang M, Xu C, Zhao Y, Xian P, Li Y, Zheng W, Yi X, Wu S, Wang Y.
USA), mouse anti-CaMKII antibody (Cat.
ab22609
,
1:100
,
Abcam
, Cambridge, UK), rabbit anti-Mash1 (Cat.
ab74065
,
1:100
,
Abcam
, Cambridge, UK), rabbit anti-
VGLUT2
(Cat.
71555
,
1:100
,
Cell signaling technology
, Danvers,
(5)
Elevated ASCL1 activity creates de novo regulatory elements associated with neuronal differentiation
BMC Genomics
December 1, 2022
Laura M. Woods, Fahad R. Ali, Roshna Gomez, Igor Chernukhin, Daniel Marcos, Lydia M. Parkinson, Ahmad N. Abou Tayoun, Jason S. Carroll, Anna Philpott
and blocked with 5% milk 0.1% TBS-Tween 20 for 1 h, before probing with anti-ASCL1 1 in 1000 (
Abcam
,
ab74065
). or anti-α-tubulin 1 in 5000 (
Abcam
ab7291
). For Phos-tag™
western blot
, phosphatase treatment was
(6)
Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes
Cell stem cell
October 6, 2022
Wang H, Keepers B, Qian Y, Xie Y, Colon M, Liu J, Qian L.
Anti-
MEF2C
antibody,
Abcam
,"
ab211493
, AB_2864417"
Anti-MASH1/Achaete-scute homolog 1
antibody,
Abcam
,"
ab74065
, AB_1859937"
Anti-beta III Tubulin
antibody,
Abcam
,"
ab7751
, AB_306045" Recombinant Anti-Albumin antibody,
Abcam
,"
ab207327
,
(7)
The Lineage Differentiation and Dynamic Heterogeneity of Thymic Epithelial Cells During Thymus Organogenesis
Frontiers in Immunology
February 22, 2022
Hanchao Gao et al.
(Cat#) Antibodies,, PerCP Cy5.5 anti-mouse Cd45,
BioLegend
,
147706
Anti-mouse
Aqp5
,
Santa Cruz
,
sc-514022
Anti Ascl1,
Abcam
,
ab74065
Anti
Cdx1
,
Abclonal
,
A5712
Anti
Hnf1a
,
Abclonal
,
A3092
Anti-mouse
Aire
,
eBioscience
,
14-5934-82
AF488
(8)
ASCL1 phosphorylation and ID2 upregulation are roadblocks to glioblastoma stem cell differentiation
Scientific reports
February 11, 2022
Azzarelli R, McNally A, Dell'Amico C, Onorati M, Simons B, Philpott A.
in 0.01% Triton X100—PBS and incubated with the primary antibodies: rabbit anti-
ASCL1
1:200
(
Abcam
,
ab74065
),
mouse
anti-TUBB3,
1:1000
(Covance MMS-435P),
chicken
anti-TUBB3,
1:1000
(
Abcam
ab41489
)
mouse
anti-
MAP2
(9)
ASCL1 and DLL3 expressions and their clinicopathological implications in surgically resected pure small cell lung cancer: A study of 247 cases from the National Cancer Center of China
Thoracic Cancer
February 1, 2022
Chunfang Hu et al.
performed on
formalin‐fixed paraffin‐embedded
(FFPE) tissues during routine clinical practices. ASCL1 (
ab74065
,
Abcam
) and DLL3 (E3J5R, Cell Signal Technology)
staining
was examined on SCLC tissue microarrays. The
(10)
c-Myc Targets HDAC3 to Suppress NKG2DL Expression and Innate Immune Response in N-Type SCLC through Histone Deacetylation
Cancers
January 18, 2022
Zhao P, Sun X, Li H, Liu Y, Cui Y, Tian L, Cheng Y.
Chicago, IL, USA), NeuroD1 (
4373S
,
CST
, Boston, MA, USA),
YAP1
(
14074S
,
CST
, Boston, MA, USA), ASCL1 (
ab74065
,
Abcam
, Cambridge, UK), HDAC1 (WL01297, Wanleibio, Shenyang, China), HDAC2 (WL03149, Wanleibio, Shenyang,
(11)
Foxa2 and Pet1 Direct and Indirect Synergy Drive Serotonergic Neuronal Differentiation
Frontiers in neuroscience
January 1, 2022
Aydin B, Sierk M, Moreno-Estelles M, Tejavibulya L, Kumar N, Flames N, Mahony S, Mazzoni EO.
antibodies. 5 μg of the following antibodies were used for
immunoprecipitation
: anti-Ascl1 (
Abcam
,
ab74065
); anti-HA (
Abcam
,
ab9110
); anti-V5 (
Abcam
,
ab15828
). Subsequent washes were done in 1X
sonication
buffer
(12)
Ascl1 phospho-site mutations enhance neuronal conversion of adult cortical astrocytes in vivo
Frontiers in neuroscience
January 1, 2022
Ghazale H, Park E, Vasan L, Mester J, Saleh F, Trevisiol A, Zinyk D, Chinchalongporn V, Liu M, Fleming T,
et al
.
anti-β-actin (#
ab8227
,
Abcam
), 1/1000
mouse
anti-
ASCL1
(#
556604
, BD Biosciences), 1/1000 rabbit pAb (#
ab74065
,
Abcam
) anti-ASCL1and Rabbit mAb anti-phospho-
Ascl1
(Li et al., 2014).
Densitometry
was assessed using
(13)
Sox2 Is an Oncogenic Driver of Small-Cell Lung Cancer and Promotes the Classic Neuroendocrine Subtype
Molecular cancer research : MCR
December 1, 2021
Voigt E, Wallenburg M, Wollenzien H, Thompson E, Kumar K, Feiner J, McNally M, Friesen H, Mukherjee M, Afeworki Y,
et al
.
1:500
), cleaved
caspase 3
(
CC3
,
Cell Signaling
9664
,
1:100
), ki67 (Biocare CRM325,
1:100
), ASCL1 (
Abcam
ab74065
,
1:500
), and
MYC
(
c-MYC
,
Invitrogen
MA1-980
,
1:100
) (Supplemental Table S4). To computationally assess
(14)
Subtype heterogeneity and epigenetic convergence in neuroendocrine prostate cancer
Nature Communications
December 1, 2021
Paloma Cejas et al.
with 1 μg of H3K27Ac antibody (
Diagenode
catalog number
C15410196), 10
μg of
ASCL1
antibody (
Abcam
ab74065
), or 10 μg of
NEUROD1
antibody (
Cell Signaling
mAb #
4373
). Five micrograms of
chromatin
was used for
(15)
An interpretable bimodal neural network characterizes the sequence and preexisting chromatin predictors of induced transcription factor binding
Genome Biology
December 1, 2021
Divyanshi Srivastava, Begüm Aydin, Esteban O. Mazzoni, Shaun Mahony
Ascl1 and Neurog2 binding was assayed by
ChIP-seq
12 h after Dox induction using the anti-Ascl1 (
Abcam
,
ab74065
) and anti-Neurog2 (
Santa Cruz
,
SC-19233
) antibodies. We assayed histone modifications as well as
chromatin
(16)
Transcription factor-based gene therapy to treat glioblastoma through direct neuronal conversion
Cancer biology & medicine
March 23, 2021
Wang X, Pei Z, Hossain A, Bai Y, Chen G.
anti-
NeuroD1
Mouse
1:1,000
Abcam
AB60704
Polyclonal anti-Neurog2 Rabbit
1:600
Abcam
AB154293
Polyclonal anti-Ascl1 Rabbit
1:800
Abcam
AB74065
Cancer
Biol Med Vol 18, No 3 August 2021 Table S2 Sequences of primers used in
RT-PCR
. Primers Sequence DCX-F TGCTTGGGCCTCACACTAGC DCX-R CATATACCGCAATCAAGGAAATACTC Ascl1-F GTCAAGTTGGTCAACCTGGG Ascl1-R CTCATCTTCTTGTTGGCCGC NEUROD2-F TCAGACATGGACTATTGGCAG NEUROD2-R GGGACAGGAAAGGGAACC
NEUROG2
-F ATTTGCAATGGCTGGCATCT
NEUROG2
-R CACAGCCTGCAGACAGCAAT
NEUROD1
-F CCTGCAACTCAATCCTCGGA
NEUROD1
-R GGCATGTCCTGGTTCTGCTC
GAPDH
-F TGGGCTACACTGAGCACCAG
GAPDH
-R GGGTGTCGCTGTTGAAGTCA ASCL1-F CAACGACTTGAACTCCATGGC ASCL1-R TTGGTGAAGTCGAGAAGCTCC DLX2-F CAACAACGAGCCTGAGAAGGAG DLX2-R GGAAACTGGAGTAGATGGTGCG pipettes
(17)
Clinicopathological features and prognostic implications of ASCL1 expression in surgically resected small cell lung cancer
Thoracic Cancer
January 1, 2021
Jiacong Wei, Li Liu, Yiying Guo, Jinyao Zhang, Xin Wang, Jiyan Dong, Puyuan Xing, Jianming Ying, Lin Yang, Junling Li
re‐sections of the cores were supplemented. The rabbit polyclonal antibody against
human
ASCL1/MASH1 (
1:200
;
ab74065
,
Abcam
) was pretested on sections from the
rat
brain (Beijing LongMaiDaS Co.,Ltd.) and a good negative
(18)
Reciprocal H3.3 gene editing identifies K27M and G34R mechanisms in pediatric glioma including NOTCH signaling
Communications Biology
December 1, 2020
Kuang-Yui Chen, Kelly Bush, Rachel Herndon Klein, Vanessa Cervantes, Nichole Lewis, Aasim Naqvi, Angel M. Carcaboso, Mirna Lechpammer, Paul S. Knoepfler
anti-K36me3 (
Abcam
, #
ab9050
), rabbit anti-NOTCH (
Abcam
, #
ab52627
), rabbit anti-ASCL1(MASH1) (
Abcam
, #
ab74065
),
mouse
anti-beta actin (
Sigma
, #
A1978
), rabbit anti-RBPJ (Millipore-Sigma #ABE384); secondary antibodies—IRDye
(19)
The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth
eLife
April 16, 2020
Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat MF, Poet GJ, Li C, Fan Y, Pruett-Miller SM,
et al
.
(
Cell Signaling Technology
,
34589
);
1:2000
. Rabbit α-
HDAC2
(
Cell Signaling Technology
,
57156
);
1:2000
. Rabbit α-MASH1 (
Abcam
,
ab74065
);
1:5000
.
Sheep
α-NETRIN1 (
R&D Systems
,
AF6419
);
1:1000
.
Mouse
α-PAX6 (DSHB, AB_528427);
1:500
.
Goat
(20)
hASH1 nuclear localization persists in neuroendocrine transdifferentiated prostate cancer cells, even upon reintroduction of androgen
Scientific Reports
December 13, 2019
Jennifer A. Fraser, Joseph E. Sutton, Saba Tazayoni, Isla Bruce, Amy V. Poole
(
Santa Cruz Biotechnology
,
sc-1615
), anti-CD-44 (
Cell Signalling
Technology, #
5640
); anti-hASH1 (
Abcam
,
Ab74065
); anti-NSE (
Abcam
,
Ab16808
) or anti-PSA (
1:200
;
Santa Cruz Biotechnology
,
sc-7638
) at a dilution of
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Anti-MASH1/Achaete-scute homolog 1 antibody from Abcam
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